BDBM19149 CHEMBL98::N-hydroxy-N'-phenyloctanediamide::SAHA::US10011611, SAHA::US10188756, Compound SAHA::US11207431, SAHA::US11505523, Compound SAHA::US9115116, SAHA::US9353061, SAHA::US9428447, SAHA::US9695181, Vorinostat::Vorinostat::Zolinza::cid_5311::suberoylanilide hydroxamic acid
SMILES ONC(=O)CCCCCCC(=O)Nc1ccccc1
InChI Key InChIKey=WAEXFXRVDQXREF-UHFFFAOYSA-N
Activity Spreadsheet -- Enzyme Inhibition Constant Data from BindingDB
Found 195 hits for monomerid = 19149
Affinity DataKi: 173nMAssay Description:Competitive inhibition of HDAC8 using KI-104 as substrate by fluorescence assayMore data for this Ligand-Target Pair
3D Structure (crystal)Affinity DataKi: 396nMAssay Description:Inhibition of human recombinant HDAC8 using fluorescent acetylated substrateMore data for this Ligand-Target Pair
Affinity DataKi: 480nMAssay Description:The compound was tested in vitro for binding activity against M1 muscarinic receptor in homogenates of the cerebral cortex of rat using [3H]pirenzepi...More data for this Ligand-Target Pair
Affinity DataKi: 480nMAssay Description:Inhibition of human recombinant HDAC8 by Michaelis-Menten equation analysisMore data for this Ligand-Target Pair
Affinity DataKi: 700nMAssay Description:Inhibition of human recombinant HDAC8 using [Ac-Leu-Gly-Lys(Ac)-AMC substrate incubated for 15 to 30 minsMore data for this Ligand-Target Pair
Affinity DataKi: 2.00E+3nM ΔG°: -7.69kcal/molepH: 7.4 T: 2°CAssay Description:To assess the effect of test compounds on histone deacetylase enzyme function in Vitro, a fluorometric assay was performed using HDAC, which incubate...More data for this Ligand-Target Pair
Affinity DataKi: 5.33E+3nMAssay Description:Inhibition of recombinant human HDAC8More data for this Ligand-Target Pair
Affinity DataKi: 1.57E+4nMAssay Description:Inhibition of C-terminal His-fusion tagged/N-terminal Strep-2 tagged recombinant human HDAC8 (1 to 377 residues) expressed in insect cells using Boc-...More data for this Ligand-Target Pair
Affinity DataIC50: 1.52E+3nMAssay Description:The inhibitory effects of compounds on histone deacetylase (HDAC) activity were determined using a fluorescence-based assay with electrophoretic sepa...More data for this Ligand-Target Pair
Affinity DataIC50: 1.86E+3nMT: 2°CAssay Description:The Fluor-de-Lys HDAC activity assay kit (Biomol) was used. Purified recombinant HDAC enzyme was incubated with Fluor-de-Lys substrate in the presenc...More data for this Ligand-Target Pair
Affinity DataIC50: 1.70E+3nMpH: 8.0 T: 2°CAssay Description:The Fluor-de-Lys HDAC activity assay kit (Biomol) was used. Purified recombinant HDAC enzyme was incubated with Fluor-de-Lys substrate in the presenc...More data for this Ligand-Target Pair
Affinity DataIC50: 232nMT: 2°CAssay Description:Fluor de Lys is a fluorescence based HDAC activity assay comprising a combination of fluorogenic Histone deAcetylase Lysyl substrate and a developer....More data for this Ligand-Target Pair
Affinity DataKd: 6.80E+3nMAssay Description:To determine the dissociation rate of the inhibitor, 1 µM HDAC8 and 20 µM inhibitor (syringe I) were mixed with 100 µM c-SAHA (syringe...More data for this Ligand-Target Pair
Affinity DataKd: 1.20E+3nMpH: 7.5Assay Description:A fixed concentration of 1.5 µM was titrated with an increasing concentration of the respective inhibitor in protein storage buffer containing 5...More data for this Ligand-Target Pair
Affinity DataIC50: 1.02E+3nMpH: 7.4 T: 2°CAssay Description:Purified HDAC1-9 (0.5~5 nM) were incubated with 2 μM carboxyfluorescein (FAM)-labeled acetylated peptide substrate A or B and test compound for ...More data for this Ligand-Target Pair
Affinity DataIC50: 1.10E+3nMAssay Description:Briefly, the HDAC8 enzyme was diluted 20 times. Then, 10 μL of HDAC8 solution was mixed with compounds at various concentrations (50 μL). T...More data for this Ligand-Target Pair
Affinity DataIC50: 410nMpH: 8.0 T: 2°CAssay Description:Following transfection of HEK293 cells with pBJ5-HDAC1 wild type or mutant plasmids, [Weerasinghe et al., J. Med. Chem. 51:5542-5551; Wambua et al., ...More data for this Ligand-Target Pair
Affinity DataIC50: 827nMpH: 7.5Assay Description:Biochemical assays of HDAC activity were carried out by Nanosyn in a reaction volume of 10 ul in 384-well microplates. A standard enzymatic reaction ...More data for this Ligand-Target Pair
Affinity DataIC50: 1.41E+3nMAssay Description:The nucleus extract was extracted from HDAC8 enzyme was expressed in Escherichia coli. Boc-Lys (acetyl)-AMC was used as the substrate of HDAC. SAHA w...More data for this Ligand-Target Pair
Affinity DataIC50: 306nMAssay Description:Inhibition of HDAC8 (unknown origin) using fluorogenic peptide from p53 residues (379 to 382) (RHK(Ac)K(Ac)) as substrate by fluorescence assayMore data for this Ligand-Target Pair
Affinity DataIC50: 243nMAssay Description:Inhibition of human HDAC-8 using RHK(Ac)K(Ac) as substrateMore data for this Ligand-Target Pair
Affinity DataIC50: 243nMAssay Description:Inhibition of human HDAC-8 using RHKK(Ac) as substrate by fluorescence assayMore data for this Ligand-Target Pair
Affinity DataIC50: 240nMAssay Description:Inhibition of human HDAC8 using fluorogenic tetrapeptide RHKKAc as substrateMore data for this Ligand-Target Pair
Affinity DataIC50: 243nMAssay Description:Inhibition of HDAC8 (unknown origin) using fluorogenic tetrapeptide RHK(Ac)K(Ac) substrate by fluorescence assayMore data for this Ligand-Target Pair
Affinity DataIC50: 310nMAssay Description:Inhibition of HDAC8 (unknown origin) using fluorogenic peptide as substrate by fluorescence assayMore data for this Ligand-Target Pair
Affinity DataIC50: 4.89E+3nMAssay Description:Inhibition of human recombinant HDAC8 after 60 mins by fluorimetric assayMore data for this Ligand-Target Pair
Affinity DataIC50: 1.50E+3nMAssay Description:Inhibition of full length C-terminal 6x-His tagged human HDAC8 using Arg-His-Lys(Ac)-Lys(Ac) substrate incubated for 2 hrs by fluorescence assayMore data for this Ligand-Target Pair
Affinity DataIC50: 243nMAssay Description:Inhibition of human HDAC8 using RHK(Ac)K(Ac) as substrate by fluorimetric analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 290nMAssay Description:Inhibition of Histone deacetylase 8 (HDAC8) of HeLa nuclear extractsMore data for this Ligand-Target Pair
Affinity DataIC50: 110nMAssay Description:Inhibition of human HDAC8 pre-incubated for 30 mins before substrate addition and measured after 30 mins by HDAC-Glo I/II assayMore data for this Ligand-Target Pair
Affinity DataIC50: 660nMAssay Description:Inhibition of HDAC8 (unknown origin) using RHK(Ac)K(Ac)AMC as substrateMore data for this Ligand-Target Pair
Affinity DataIC50: 232nMAssay Description:Inhibition of His-tagged HDAC8 (unknown origin) expressed in Escherichia coli BL21(DE3) after 60 mins by MALDI mass spectrometryMore data for this Ligand-Target Pair
Affinity DataIC50: 1.68E+3nMAssay Description:Inhibition of recombinant HDAC8 (unknown origin) using fluorogenic substrate Boc-Lys (acetyl)-AMC after 20 mins by homogeneous fluorescence release a...More data for this Ligand-Target Pair
Affinity DataIC50: 5.5nMAssay Description:Inhibition of full length human recombinant HDAC8 expressed in baculovirus infected insect S9 cells using Ac-Leu-GlyLys(Tfa)-AMC as substrate after 3...More data for this Ligand-Target Pair
Affinity DataIC50: 1.34E+3nMAssay Description:Inhibition of human recombinant HDAC8 using MAZ1675 as fluorogenic substrate measured every 5 mins by optimized homogenous fluorescence based assayMore data for this Ligand-Target Pair
Affinity DataIC50: 8.80nMAssay Description:Inhibition of recombinant C-terminal His-tagged full length human HDAC8 expressed in baculovirus infected insect Sf9 cells using fluorogenic HDAC cla...More data for this Ligand-Target Pair
Affinity DataIC50: 1.68E+3nMAssay Description:Inhibition of recombinant HDAC8 (unknown origin) incubated for 10 mins using Boc-Lys(acetyl)-AMC fluorogenic substrate by homogeneous fluorescence re...More data for this Ligand-Target Pair
Affinity DataIC50: 310nMAssay Description:Inhibition of HDAC8 (unknown origin) using RHK(Ac)K(Ac) fluorogenic acetylated peptide substrate by fluorometric assayMore data for this Ligand-Target Pair
Affinity DataIC50: 4.89E+3nMAssay Description:Inhibition of recombinant human HDAC8 after 60 mins by fluorescence assayMore data for this Ligand-Target Pair
Affinity DataIC50: 1.14E+3nMAssay Description:Inhibition of HDAC8 (unknown origin) after 17 hrsMore data for this Ligand-Target Pair
Affinity DataIC50: 1.38E+3nMAssay Description:Inhibition of human recombinant HDAC8More data for this Ligand-Target Pair
Affinity DataKd: 580nMAssay Description:Binding affinity to human recombinant HDAC8 by fluorescence assayMore data for this Ligand-Target Pair
Affinity DataIC50: 1.70E+3nMAssay Description:Inhibition of His-tagged HDAC8 catalytic domain (unknown origin) expressed in Escherichia coliMore data for this Ligand-Target Pair
Affinity DataIC50: 1.70E+3nMAssay Description:Inhibition of human recombinant HDAC8 by fluorometryMore data for this Ligand-Target Pair
Affinity DataIC50: 119nMAssay Description:Inhibition of HDAC8 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 1.70E+3nMAssay Description:Inhibition of C-terminal His-tagged HDAC8 expressed in Escherichia coliMore data for this Ligand-Target Pair
Affinity DataIC50: >1.00E+4nMAssay Description:Inhibition of recombinant human HDAC8 using Boc-Lys(TFA)-AMC as substrate preincubated for 15 mins followed by substrate addition measured after 60 m...More data for this Ligand-Target Pair
Affinity DataIC50: 3.70E+3nMAssay Description:Inhibition of C-terminal His-tagged human recombinant HDAC8 expressed in Sf9 cells after 30 mins by microtiter plate reader analysisMore data for this Ligand-Target Pair
Activity Spreadsheet -- ITC Data from BindingDB
Found 2 hits for monomerid = 19149
ITC DataΔG°: -8.36kcal/mole −TΔS°: 2.42kcal/mole ΔH°: -10.9kcal/mole logk: 1.36E+6
T: 25.00°C
T: 25.00°C
ITC DataΔG°: -8.36kcal/mole −TΔS°: 0.0519kcal/mole ΔH°: -8.96kcal/mole logk: 2.21E+6
pH: 7.5 T: -248.15°C
pH: 7.5 T: -248.15°C